Food safety analysis workflow
Thermo Scientific Life Science MS not only has a long tradition of technology, but also continues to innovate. Quadrupole mass analysis of true conjugate hyperboloids that fits the perfect theoretical electric field of a textbook is used on all TSQ® triple quadrupole mass spectrometers. Since launching the world's first triple quadrupole MS/MS (TSQ®) mass spectrometer in 1980, TSQ® triple quadrupole mass spectrometer has been widely used in environmental protection, agriculture, quarantine, food safety, etc. Analytical testing, fully capable of testing routine testing of large-volume samples of crops, livestock and poultry products, dairy products and related processed foods, such as pesticide residues in food, veterinary drug residues, mycotoxins, additives, nutritional supplements and organic pollutants And other projects to help you easily meet the increasingly stringent regulatory requirements.
TSQ® Triple Quadrupole Mass Spectrometer and Thermo Fisher Food Safety Testing Strategy
summary
Methylene blue is a thiazide dye. It is mainly used as a disinfectant and antidote in veterinary drugs. It is similar to triphenylmethane dyes such as malachite green and crystal violet, which are prohibited from aquaculture. Daily testing involves aquatic products and feed. Medium sample. This article is simplified from SN/T 1974-2007 and is an instrumental method for the rapid detection of methylene blue and its three metabolite residues, quantified by external standard method.
Pretreatment
For related reagent materials, standard configuration and reserves, and sample preparation methods, please refer to the above standards. For related instrument methods, please refer to this document. ( TSQ Triple Quadrupole Mass Spectrometer Concise Application Manual - Food Safety Testing )
instrument
The TSQ Triple Quadrupole LC/MS system is equipped with an ultra-high pressure liquid phase system.
Liquid phase condition
Mass spectrometry condition
SRM condition
Experimental result
Typical chromatogram
in conclusion
In the above national standard detection method, the detection limit of methylene blue in different matrices was 5 μg/kg. In addition to methylene blue, the method includes three metabolites of methylene blue, which fully meet the requirements of domestic and international regulations for sensitivity and reproducibility of conventional regulatory methods.
