Amino black 10B / naphthol blue black / buffalo black NBR / acid black 1 / aniline blue black / acid black 10B / acid blue black 10B / acid cyan blue 10B / 4-amino-5-hydroxy-3- (p-nitro Benzoazo) -6- (Benzazo) -2,7-naphthalene disulfonic acid disodium sal

English name: Amino black 10B; Buffalo black NBR; Acid black 1
Other names: naphthol blue black; buffalo black NBR; acid black 1; aniline blue black; acid black 10B; acid blue black 10B; acid glaucoma blue 10B; 4-amino-5-hydroxy-3- (p-nitrobenzene Azo) -6- (Benzazo) -2,7-naphthalene disulfonic acid disodium salt; naphthalene blue black
CAS number: 1064-48-8
C22H14N6Na2O9S2 = 616.49
Level: High Purity Grade
Em (Lambda Max, Water):> 28500
Lambda Max (nm): 615 ～ 621
Properties: blue black or dark brown powder. Soluble in water (blue-black), ethanol (dark blue) and cellosolve (diethanol-ethyl ether), slightly soluble in acetone, insoluble in other organic solvents. In case of concentrated sulfuric acid, it is blue-green, which is diluted into dark green and blue precipitate. In case of concentrated nitric acid, it is dark green solution. Blue solution with 10% sodium hydroxide solution showing red fluorescence. Concentrated hydrochloric acid is added to the aqueous solution to produce a green-blue precipitate, and concentrated sodium hydroxide solution is added to form a blue precipitate. When dyeing, it encounters copper; iron ions, the color of the former changes slightly, the latter is slightly greenish Use: Biochemical research. Electrophoresis reagent for staining proteins on polyacrylamide gel, agarose gel and nitrocellulose membrane. After electrophoresis, it is recommended to fix the protein on the gel. The glue is stained with 0.1% amino black 10B in 7% (v / v) acetic acid solution for at least 2 hours, and then decolorized with 7% (v / v) acetic acid solution. The detection sensitivity is about 20% of the Coomassie Brilliant Blue R250. Redox indicator storage: RT